Siwa, who recently said she uses any pronouns, has been bonding with fellow housemate, Love Island UK star Chris Hughes, and was sending him private messages, which led to the two of them being called into the Diary Room.
There, producers asked about the private messages, and Siwa came forward, saying the messages were about her exploring her identity.
"It's something that I'm not ready, necessarily, to be open about or up front about, but it has to do with gender and 'how do I feel?'"
Let's put it this way – oh my God, this is a lot, this is a lot," Siwa said, as Hughes comforted them.
"Essentially, you have female, you have male. I've met a lot of females, love them, don't feel like I'm them. Met a lot of males, love them, definitely not them," Siwa continued. "Met a lot of people in my life that are nonbinary, and these beautiful nonbinary people are who I feel the most like."
They said that defining a gender identity is "not something I want to say about myself yet," but they are having confusing thoughts about it.
"JoJo, thank you for being so honest and sharing with Big Brother," Hughes said, putting his arm around Siwa.
"It's important to be honest, and I think it's important to show that it's okay to not know," Siwa said.
Later, Siwa spoke to other housemates about what she said, telling them she shouldn't be scared about talking about this, but because she doesn't have all the answers, she is.
"You're growing up in a public spotlight, you don't need to give anyone all the answers, ever. Ever. Even when you know them for you, you don't owe anyone anything ever," housemate Danny Beard, a drag queen, told Siwa.
"As long as you know who you are, and what have you, the truth is, it might hurt, people might judge you, but if the people you know and you love and your community amd your support know the truth, today's headlines, as they used to say, is tomorrow's loo paper," added housemate Trisha Goddard.
Siwa had previously said in her introduction on the show that her pronouns are "anything you feel on that day, whatever I look like, you can call me."
The 21-year-old singer came out as pansexual in 2021, and has dated women and nonbinary people, since then.